Fred Asiegbu
University of Helsinki
Contact by mailBIOGRAPHICAL SKETCH NAME: Fred O. Asiegbu POSITION TITLE: Professor, University of Helsinki Finland EDUCATION/TRAINING University of Agriculture, Uppsala (Sweden) Docent 1998 Forest Microbiology University of Stathclyde, Glasgow (UK) PhD 1992 Biotechnology University of Jos (Nigeria) MSc 1985 Microbiology/PlantPathology University of Benin (Nigeria) BSc 1983 Botany B. Positions and Honors. CURRENT APPOINTMENT 2007 (January) to date: Professor of Forest Pathology, University of Helsinki, Finland PRIOR EXPERIENCE 1998 - 2006: Associate Professor (Docent) in Forest Microbiology and Pathology, SLU, Uppsala 1996: Research Associate, Department of Forest Mycology & Pathology, SLU, Uppsala 1995 – 1996 : Postdoctoral scientist, Department of Plant Pathology, Univ. of California, Riverside, USA. 1991 – 1994 : Postdoctoral scientist, Department of Forest Mycology and Pathology, SLU, Uppsala PROFESSIONAL ACTIVITIES -External evaluator International Foundation for Science (IFS), -Reviewer of grant applications for Canadian Forestry Research Council. -Member of the evaluation and grading committee for 5-Ph.D students. - Evaluator for university lecturer position in Forest pathology, Helsinki University -Evaluator for Austrian National Research Council -Evaluator for National Science Foundation (NSF, USA). -Member of the Biotechnology board at Helsinki University, with resposibility for "MBiot" students with interest in Forest ecology, Forest Microbiology, Forest Pathology and Tree health biotechnology -deputy member of the board for International masters programme in forestry and Business in the faculty of Agriculture and Forestry, -Member of the newly constituted board for Helsinki University International Forum. -Member of "ATLANTIS" Board - Transatlantic Master’s degree program in Forest Resources. -Member of the board on Biological Interaction Graduate school, Finland -Referee/Reviewer for several journals -Member European union cost action group on endophytes in Biotechnology -Member European Union cost action on Pine Strength -Member editorial board of Applied and Environmental microbiology -Member Editorial board of Biodiversity Management and Forestry -Member Editorial board of Phytopathologia Polonica 3. GRADUATE STUDENT AND POST-DOC SUPERVISION (postdoc, doctoral dissertations & licentiates) a. POSTDOC SUPERVISION :Have supervised a total of 10 postdocs to date and currently advising 7 postdocs. b. Ph.D student supervision: Have supervised a total of 20 doctoral students; 12 have defended their thesis, currently supervising 6 as main and 2 as deputy supervisor. c. M.Phil (Licentiate): Have supervised two licentiate thesis d. M.Sc STUDENT'S SUPERVISION: have supervised 9 master’s thesis and currently supervising three other students. 4a. PUBLICATIONS; ca120 publications in international refereed journals, books and extended abstracts in conference proceedings. 4b. Presentation at International meetings and national conferences: ca 78 abstracts in conference presentations at various national and international scientific meetings. 4c. SELECTED PUBLICATIONS (Refereed journal publications) 1. ASIEGBU FO, DANIEL G & JOHANSSON M. 1993. Studies on infection of Norway spruce roots by Heterobasidion annosum. Canadian Journal of Botany 71: 1552 - 1561. 2. ASIEGBU FO, MORISSON I, PATERSON A & SMITH JE 1994a. Analyses of fungal fermentation of lignocellulosic substrates using continuos culture rumen simulation. Journal of General and Applied Microbiology 40: 305 - 318. 3. ASIEGBU FO, DANIEL G & JOHANSSON M. 1994b. Defence related reactions of seedling roots of Norway spruce to infection by Heterobasidion annosum (Fr.) Bref. Physiological and Molecular Plant Pathology 45: 1 - 19. 4. ASIEGBU FO, DENEKAMP M, DANIEL G & JOHANSSON M. 1995a. Immunocytochemical localisation of pathogenesis related proteins in roots of Norway spruce infected with Heterobasidion annosum. European Journal of Forest Pathology 25 (3): 161 - 170. 5. ASIEGBU FO, DANIEL G & JOHANSSON M. 1995b. Infection and disintegration of vascular tissues of non-suberized roots of spruce by Heterobasidion annosum and use of antibodies for characterising infection. Mycopathologia 129: 91 - 101. 6. ASIEGBU FO, MORISSON I, PATERSON A & SMITH JE 1995c. Effect of plant cell wall phenolics and fungal metabolites on acetate and methane production under in vitro rumen conditions. Journal of General and Applied Microbiology 41: 475 - 485. 7. ASIEGBU FO, PATERSON A & SMITH JE 1996a. Inhibition of cellulose saccharification and glyco-lignin attacking enzymes of five lignocellulose degrading fungi by ferulic acid. World Journal of Microbiology and Biotechnology 12 (1): 16 - 21. 8. ASIEGBU FO, DANIEL G & JOHANSSON M. 1996b. Cellular Interaction between the saprotroph Phlebiopsis gigantea and non-suberized roots of Picea abies. Mycological Research 100(4): 409 - 417. 9. ASIEGBU FO, PATERSON A & SMITH JE 1996c. The effects of co-fungal cultures and supplementation with carbohydrate adjuncts on lignin biodegradation and substrate digestibility. World Journal of Microbiology and Biotechnology 12: 273 - 279. 10. ASIEGBU FO, LÖNNEBORG A, & JOHANSSON M. 1996d. Chitins and glucans detected in cell walls of the oomycetous fungus-Pythium dimorphum. European Journal of Forest Pathology 6: 315 - 321. 11. ASIEGBU FO, JOHANSSON M & DANIEL G. 1997. Cytopathological response reactions of Picea abies roots to infection by the necrotroph- Fusarium avenaceum (Fr.) Sacc.) Journal of Phytopathology 145: 239 - 244. 12. DANIEL G, ASIEGBU FO & JOHANSSON M. 1998. Saprophytic wood decaying capabilities of the S and P intersterility groups of Heterobasidion annosum. Mycological Research 102: 991 – 998. 13. JOHANSSON M, LUNDGREN L & ASIEGBU FO 1998. Differential phenol-induced laccase activity of the S and P intersterility groups of the conifer root pathogen Heterobasidion annosum. Microbiological Research 153: 71 – 80. 14. ASIEGBU FO, JOHANSSON M, HUTTERMANN A & WOODWARD S 1998. Biochemistry of the host-parasite interaction. In Heterobasidion annosum in European Forests: Prospects for Control. Edited by S. Woodward, J. Stenlid, R. Karjalainen & A. Huttermann. CAB International Publishers, London pp167 - 193. 15. JOHANSSON M, DENEKAMP M & ASIEGBU FO 1999. Production and isozyme pattern of extracellular laccase in the S and P intersterility groups of the root pathogen Heterobasidion annosum. Mycological Research 103 (3): 365 – 371. 16. ASIEGBU FO, JOHANSSON M & STENLID J. 1999a. Reactions of Pinus sylvestris (Scots pine) root tissues to the presence of mutualistic, saprotrophic and necrotrophic micro-organisms. Journal of Phytopathology 147: 257 – 264. 17. ASIEGBU FO, KACPRZAK, M. DANIEL, G. JOHANSSON M , STENLID J. & MANKA, M. 1999b. Biochemical interactions of conifer seedling roots with Fusarium spp Canadian Journal of Microbiology 45: 923 - 935. 18. ASIEGBU FO 2000a. Effects of ethanol, cell wall phenolics and carbohydrates on in vitro growth of necrotrophic fungi- Heterobasidion annosum and Fusarium avenaceum. Journal of Basic Microbiology 3: 139 - 148. 19. ASIEGBU FO 2000b. Adhesion and development of the root rot fungus (Heterobasidion annosum) on conifer tissues: effects of spore and host surface constituents. FEMS Microbiology Ecology 33(2): 101 - 110. 20. KACPRZAK, M. ASIEGBU FO, DANIEL G & MANKA, M. 2000. Changes in root structure of Scots pine seedlings infected with Rhizoctonia solani observed under fluorescence and electron microscopes. Phytopathologia polonica 19: 127 – 140. 21. ASIEGBU FO 2000. Genetic transformation of the root rot fungus Heterobasidion annosum by particle bombardment. Forestry Studies 34; 9 - 16. 22. KACPRZAK, M. ASIEGBU FO, & MANKA, M. 2000. Acidity and peroxidase activity change in Scots pine and Norway spruce seedling roots after infection with Rhizoctonia solani and Fusarium spp. SYLWAN 4: 99 –103. 23. KACPRZAK, M. ASIEGBU FO, & MANKA, M. 2000. Cell wall lignification in roots of Scots pine, Norway spruce and European larch seedlings after infection with Rhizoctonia solani and Fusarium spp. SYLWAN 4: 105 – 112. 24. KACPRZAK M, ASIEGBU FO, DANIEL, G. JOHANSSON M , STENLID J. & MANKA, M. 2001. Differential resistance of conifer species (European Larch, Norway spruce, Scots pine) to infection by necrotrophic parasites. European Journal of Plant Pathology 107 (2): 191 - 207. 25. NAHALKOVA J, ASIEGBU FO, DANIEL G, HRIB J, VOOKOVA B, PRIBULOVA, B & GEMEINER P. 2001. Isolation and characterization of a Pinus nigra lectin (PNL) during interaction with the necrotrophs-Heterobasidion annosum and Fusarium avanaceum. Physiological and Molecular Plant Pathology 59: 153 - 163 26. JOHANSSON M, ASIEGBU FO & PRATT J. 2002. Stump treatment against Heterobasidion annosum with urea: possible modes of action. Forest Ecology and Management- 157 (1-3): 87 - 100. 27. KACPRZAK M, ASIEGBU FO, DANIEL G, MANKA M & STENLID J. 2002. In vitro Scots pine seedling root colonization by damping-off fungi in the presence of a Trichoderma harzianum isolate. Phytopathologia. polonica. 23: 5 – 15. 28. ASIEGBU FO, CHOI W, LI G, NAHALKOVA J & DEAN RA. 2003. Isolation of a novel antimicrobial peptide gene from Pinus sylvestris. FEMS Microbiology Letters 228: 27 – 31. 29. ASIEGBU FO, CHOI W, JEONG YS & DEAN RA. 2004a. Cloning, sequencing and functional analyses of Magnaporthe grisea MVP1 gene, a hex-1 homolog encoding putative woronin body protein. FEMS Microbiology Letters 230: 85 – 90. 30. ASIEGBU FO, ABU S, STENLID J & JOHANSSON M 2004b. Molecular characterization of laccase genes of the conifer pathogen. Mycological Research 108: 136 - 148. 31. ABU SM, LI G & ASIEGBU FO 2004. Identification of Heterobasidion annosum genes involve in conidiospore germination under varying culture conditions. FEMS Microbiology Letters 233: 205 – 213. 32. LI G & ASIEGBU FO 2004 Use of Scots pine seedling roots as an experimental model to investigate gene expression during interaction with the conifer pathogen Heterobasidion annosum (P-type). Journal of Plant Research 117 (2): 155 - 162 33. JOHANSSON SM, LUNDGREN LN & ASIEGBU FO 2004. Initial reactions in sapwood of Norway spruce and Scots pine after wounding and infection by Heterobasidion parviporum and H. annosum. Forest Pathology 34 (3): 197 – 210. 34. LI G & ASIEGBU FO 2004. Induction of Pinus sylvestris PsACRE,, a homolog of Avr9/Cf-9 rapidly elicited defence related gene following infection with the root rot fungus Heterobasidion annosum. Plant Science 167: 535 - 540. 35. ASIEGBU FO, NAHALKOVA J & LI G. 2005a. Pathogen inducible cDNAs from the interaction of the root rot fungus Heterobasidion annosum (P-type) with its conifer host (Pinus sylvestris). Plant Science 168: 365 – 372. 36. ASIEGBU FO & STENLID J. 2005. Obituary: Martin Sten Johansson 1930 – 2004. Forest Pathology 34: 73 – 74. 37. ASIEGBU FO, ADOMAS A & STENLID J. 2005b. Pathogen Profile: The root and butt rot fungus-Heterobasidion annosum Molecular Plant Pathology 6(4): 395 - 409. 38. STEPNIEWSKA S, MANKA M, ASIEGBU FO 2006. Anastomosis grouping, pathogenicity and population structure of Rhizoctonia spp. isolates from forest nurseries in Poland. Forest Pathology 36: 97 - 109. 39. ADOMAS A, EKLUND M, JOHANSSON M, ASIEGBU FO 2006. Identification and analysis of differentially expressed cDNA’s during non-self competitive interaction between Phlebiopsis gigantea and Heterobasidion parviporum. FEMS Microbiology Ecology 57: 26 – 39. 40. LI G, OSBORNE J & ASIEGBU FO 2006. A macro-array expression analysis of novel cDNA’s vital for growth initiation and primary metabolism during development of Heterobasidion parviporum conidiospores. Environmental Microbiology 8: 1340 – 1360. 41. ADOMAS A & ASIEGBU FO. 2007. Analysis of organ specific responses of Pinus sylvestris to shoot (Gremmeniella abietina) and root (Heterobasidion annosum) pathogens. Physiological and Molecular plant Pathology, 69. 140 - 152. 42. ADOMAS A, LI G, ADOMAS A, CRAIG D, CHU T, HELLER G, OLSON A, ZYL LV, WOLFINGER R, SEDEROFF R, DEAN RA, FINLAY R, STENLID J, ASIEGBU FO. 2007. Transcriptome analyses of early events during interactions of Scots pine root tissues with pathogenic fungi. Tree Physiology 27, 1441–1458. 43. ADOMAS A, HELLER, G, LI G, CRAIG D, CHU T, OLSON A, ZYL LV, WOLFINGER R, SEDEROFF R, FINLAY R, STENLID J, & ASIEGBU FO. 2008. Comparative analysis of transcript abundance in Pinus sylvestris roots after challenge with a saprotrophic, pathogenic or mutualistic fungus. Tree Physiology 28, 885-897. 44. HELLER, G, ADOMAS A, LI G, ADOMAS A, CRAIG D, CHU T, ZYL LV, WOLFINGER R, SEDEROFF R, FINLAY R, STENLID J, & ASIEGBU FO. 2008. Transcriptional analysis of Pinus sylvestris roots challenged with the ectomycorrhizal fungus Laccaria bicolor. BMC Plant Biology 8: 19 - 45. LUNDEN K, EKLUND M, FINLAY R, STENLID J, ASIEGBU FO 2008. Heterologous array analysis in Heterobasidion spp. of cDNA arrays with probe from mycelium of S, P or F types. Journal of Microbiology Methods 75, 219 - 224. 46. LEHR N, ADOMAS A, ASIEGBU FO, AND HAMPP R TARKKA MT (2009) WS-5995 B, an antifungal agent inducing differential gene expression in the conifer pathogen Heterobasidion annosum. Applied Microbiology & Biotechnology 85 (2), 347 – 358. 47. SUN H, KORHONEN K, HANTULA J, ASIEGBU FO AND KASANEN R (2009). Use of a breeding approach for improving biocontrol efficacy of Phlebiopsis gigantea strains against Heterobasidion infection of Norway spruce stumps. FEMS Microbiology Ecology 69(2), 266 - 273 48. LU J, ARONEN T, PAPPINEN A AND ASIEGBU FO (2010). Response of somatic embryos of Scots pine to fungal cell wall elicitors. Forest Pathology ( http://www3.interscience.wiley.com/journal/123337753/abstract). Forest Pathology. 41 (1), 75-82 49. MGBEAHURUIKE AC, SUN H, FRANSSON P, KASANEN R, DANIEL G, KARLSSON M AND ASIEGBU FO (2011). Differential screening of Phlebiopsis gigantea isolates for traits associated with biocontrol of the conifer pathogen Heterobasidion annosum. Biological Control: 57, 118 - 129. 50. SUN H, PAULIN L, ALATALO E, ASIEGBU FO (2011) Response of living tissues of Pinus sylvestris to the saprotrophic biocontrol fungus Phlebiopsis gigantea. Tree Physiology 31: 438-451 51. SOORIYAARACHCHI S, JABER E, SUÁREZ COVARRUBIAS A, UBHAYASEKERA W, ASIEGBU FO, MOWBRAY S (2011) Expression and β-glucan binding properties of Scots pine (Pinus sylvestris L.) antimicrobial protein (Sp-AMP). Plant Molecular Biology 77 (1-2): 33 - 45. 52. TERHONEN E, MARCO T, SUN H, JALKANEN R, KASANEN R, VUORINEN M. ASIEGBU FO (2011). The effect of latitude, season and needle-age to the mycobiota of Scots pine (Pinus sylvestris) in Finland. Silva Fennica 45(3): 301 – 317. 53. SOORIYAARACHCHI S, SUÁREZ COVARRUBIAS A, UBHAYASEKERA W, ASIEGBU FO, MOWBRAY S (2011). A new class of Scots pine antimicrobial proteins, which act by binding b-glucan. In: SCIENCE AND TECHNOLOGY AGAINST MICROBIAL PATHOGENS Research, Development and Evaluation Proceedings of the International Conference on Antimicrobial Research (ICAR2010) Valladolid, Spain, 3 – 5 November 2010 edited by A Mendez-Vilas (Formatex Research Center, Spain). 448pp Pub. date: Jul 2011 ISBN: 978-981-4354-85-1, 981-4354-85-6 54. HELLER G, LUNDEN K, FINLAY R, ASIEGBU FO & ELFSTRAND M (2012). Expression analysis of Clavata1-like and Nodulin21-like genes from Pinus sylvestris during ectomycorrhiza formation. Mycorrhiza 22:271–277: DOI: 10.1007/s00572-011-0402-2 55. Eastwood DC, Floudas D, Binder M, Majcherczyk A, Schneider P, Aerts A, Asiegbu FO, Baker SE, Barry K, Bendiksby M, Blumentritt M, Coutinho PM, Cullen D, de Vries RP, Gathman A, Goodell B, Henrissat B, Ihrmark K, Kauserud H, Kohler A, LaButti K, Lapidus A, Lavin JL, Lee YH, Lindquist E, Lilly W, Lucas S, Morin E, Murat C, Oguiza JA, Park J, Pisabarro AG, Riley R, Rosling A, Salamov A, Schmidt O, Schmutz J, Skrede I, Stenlid J, Wiebenga A, Xie X, Kües U, Hibbett DS, Hoffmeister D, Högberg N, Martin F, Grigoriev IV and Watkinson SC (2011). The Plant Cell Wall-Decomposing Machinery Underlies the Functional Diversity of Forest Fungi. Science, DOI: 10.1126/science.1205411 ( Science 333: 762 - 765) 56. Åke Olson, Andrea Aerts, Fred Asiegbu, Lassaad Belbahri, Ourdia Bouzid, Anders Broberg, Björn Canbäck, Pedro M. Coutinho, Dan Cullen, Kerstin Dalman, Giuliana Deflorio, Linda T.A. van Diepen, Christophe Dunand, Sébastien Duplessis, Mikael Durling, Paolo Gonthier, Jane Grimwood, Carl Gunnar Fossdal, David Hansson, Bernard Henrissat, Ari Hietala, Kajsa Himmelstrand, Dirk Hoffmeister, Nils Högberg, Timothy Y. James, Magnus Karlsson, Mårten Lind, Annegret Kohler, Ursula Kües, Yong-Hwan Lee, Yao-Cheng Lin, Erika Lindquist, Vincent Lombard, Susan Lucas, Karl Lundén, Emmanuelle Morin, Claude Murat, Jongsun Park, Tommaso Raffaello, Pierre Rouzé, Asaf Salamov, Jeremy Schmutz, Halvor Solheim, Jerry Ståhlberg, Heriberto Vélëz, Ronald P. de Vries, Ad Wiebenga, Steve Woodward, Igor Yakovlev, Matteo Garbelotto, Francis Martin, Igor V. Grigoriev, Jan Stenlid (2012). Trade-off between wood decay and parasitism: Insights from the genome of a fungal forest pathogen. New Phytologist : 194:1001-1013 57. RAFFAELLO T, KERIÖ S AND ASIEGBU FO (2012). Role of the HaHOG1 MAP Kinase in Response of the Conifer Root and But Rot Pathogen (Heterobasidion annosum) to Osmotic and Oxidative Stress. PLOS ONE 7(2): e31186. doi:10.1371/journal.pone.0031186 58. MGBEAHURUIKE AC, KARLSSON M AND ASIEGBU FO (2012). Two hydrophobins from the biological control agent Phlebiopsis gigantea are differentially regulated by growth substrate. Fungal Biology 116:620-629 59. SUN H, TERHONEN E, KOSKINEN K, PAULIN L, KASANEN R AND ASIEGBU FO (2013) The impacts of treatment with biocontrol fungus (Phlebiopsis gigantea) on bacteria biota of Norway spruce stumps. Biological Control, 64: 238–246 http://dx.doi.org/10.1016/j.biocontrol.2012.11.015, 60. ARNERUP J, NEMESIO-GORRIZ M, LUNDÉN K, ASIEGBU FO, STENLID J AND ELFSTRAND M (2013). The primary module in Norway spruce defence signalling against H. annosum s.l. seems to be jasmonate-mediated signalling without antagonism of salicylate-mediated signalling. Planta 237: 1037–1045 (DOI: 10.1007/s00425-012-1822-8) 61. KOVALCHUK A, KERIÖ S, JABER E, OGHENEKARO A, RAFFAELLO T AND ASIEGBU FO (2013). Antimicrobial defences and resistance of forest trees: challenges and perspectives in a genomic era. Annual Review of Phytopathology 51: 221 - 244. 62. RAFFAELLO T AND ASIEGBU FO (2013) Evaluation of potential reference genes for use in gene expression studies in the conifer pathogen Heterobasidion annosum. Molecular Biology Reports 40: 4605–4611 (DOI 10.1007/s11033-013-2553-z). 63. MGBEAHURUIKE AC, KOHLER A AND ASIEGBU FO (2013). Expression analysis of the impact of culture filtrate from the biocontrol agent (Phlebiopsis gigantea) on the conifer pathogen (Heterobasidion annosum s.s.) transcriptome. Microbial Ecology 66: 669 – 681 (DOI 10.1007/s00248-013-0255-7). 64. KOVALCHUK A, LEE YH AND ASIEGBU FO (2013). Diversity and evolution of ABC proteins in basidiomycetes. Mycologia 105:1456-1470 (doi: 10.3852/13-001). 65. CHEN H, KOVALCHUK A AND ASIEGBU FO (2013). Distribution and bioinformatic analysis of cerato-platanin protein family in Dikarya. Mycologia 105:1479-1488 (doi: 10.3852/13-115). 66. MGBEAHURUIKE AC, KOVALCHUK A AND ASIEGBU FO (2013). Comparative genomics and evolutionary analysis of three species of wood degrading fungi. Mycologia 105:1471-1478 (doi: 10.3852/13-077) 67. JAMES TY, SUN S, LI W, HEITMAN J, KUO HC, ASIEGBU FO AND OLSON Å (2013). Polyporales genomes reveal the genetic architecture underlying tetrapolar and bipolar mating systems Mycologia 105:1374-1390 (doi: 10.3852/13-162 ) 68. TERHONEN E, SUN H, BUÉE M, KASANEN R, PAULIN L AND ASIEGBU F.O (2013). Effects of the use of biocontrol agent (Phlebiopsis gigantea) on fungal communities of Picea abies stumps. Forest Ecology & Management 310: 428 - 433. 69. MGBEAHURUIKE AC, KOVALCHUK A, CHEN H, UBHAYASEKERA W, ASIEGBU FO (2013). Evolutionary analysis of hydrophobin gene family in two wood-degrading basidiomycetes, Phlebia brevispora and Heterobasidion annosum s.l. BMC Evolutionary Biology (1):240. DOI: 10.1186/1471-2148-13-240 70. RAFFAELLO T, CHEN H, KOHLER A AND ASIEGBU FO (2014). Transcriptomic profiles of Heterobasidion annosum under abiotic stresses and during saprotrophic growth in bark, sapwood and heartwood. Environmental Microbiology 16 (6): 1654-67 (DOI: 10.1186/1471-2148-13-240) 71. JABER E, XIAO C AND ASIEGBU FO (2014). Comparative pathobiology of Heterobasidion annosum during challenge on Pinus sylvestris and Arabidopsis roots: analysis of defensin gene expression in the pathosystems. Planta 239: 717 – 733 (http://dx.doi.org/10.1007/s00425-013-2012-z) . 72. SUN H, TERHONEN E, KOSKINEN K, PAULIN L, KASANEN R, ASIEGBU FO (2014). Bacterial diversity and community structure along different peat soils in boreal forest. Applied Soil Ecology 74: 37 – 45. 73. SUN H, TERHONEN E, KASANEN R, ASIEGBU FO (2014). Diversity and community structure of bacteria associated with decaying wood in boreal forest. Geomicrobiology 31(4): 315 - 324 74. TERHONEN E, SUSANNA K, SUN H, AND ASIEGBU FO (2014).. Endophytic fungi of Norway spruce roots in boreal pristine mire, drained peatland and mineral soil and inhibitory effect on Heterobasidion parviporum in vitro. Fungal Ecology 9: 17 - 26. 75. OGHENEKARO AO, MIETTINEN O, OMORUSI VI, EVUEH G, FARID MA, GAZIS R, AND ASIEGBU FO (2014). Characterization of Rigidoporus species associated with white rot of Para rubber (Hevea brasiliensis) plantations Fungal Biology 118(5-6): 495-506, http://dx.doi.org/10.1016/j.funbio.2014.04.001 76. CHOI J, DÉTRY N, KIM NK, ASIEGBU FO, VALKONEN JPT AND LEE YH (2014). Fungal peroxidase database for comparative genomics. BMC Microbiology 14:117 doi:10.1186/1471-2180-14-117 77. KUO HC, CHOI J, SUN H, ASIEGBU FO, VALKONEN JPT AND LEE YH (2014). A secret life of Neurospora crassa. Scientific Reports (Nature) 4 (5135): 1 – 6, doi:10.1038/srep05135 78. DÉTRY N, CHOI J, KUO HS, ASIEGBU FO, VALKONEN JPT AND LEE YH (2014). In silico sequence analysis reveals new characteristics of fungal NADPH oxidase genes. Mycobiology 42(3) : 241-248 79. CHOI J, KIM KT, JEON J, WU J, SONG H, ASIEGBU FO AND LEE YH (2014). funRNA: a fungi-centered genomics platform for genes encoding key components of RNAI. BMC Genomics 15 (Suppl 9):S14 doi:10.1186/1471-2164-15-S9-S14 80. Chiaki Hori; Takuya Ishida; Kiyohiko Igarashi; Masahiro Samejima; Hitoshi Suzuki; Emma Master; Patricia Ferreira; Francisco J Ruiz-Duenas; Benjamin Held; Paulo Canessa; Luis F Larrondo; Monika Schmoll; Irina S Druzhinina; Christian P Kubicek; Jill Gaskell; Phil Kersten; Franz St. John; Jeremy Glasner; Grzegorz Sabat; Sandra Splinter BonDurant; Khajamohiddin Syed; Jagjit Yadav; Anthony C Mgbeahuruike; Andriy Kovalchuk; Fred O Asiegbu; Gerald Lackner; Dirk Hoffmeister; Hui Sun; Erika Lindquist; Kerrie Barry; Robert Riley; Igor V Grigoriev; Bernard Henrissat; Ursula Kües; Randy M Berka; Angel T Martinez; Sarah F Covert; Robert A Blanchette; Daniel Cullen (2014). Analysis of the Phlebiopsis gigantea genome, transcriptome and secretome gives insight into its pioneer colonization strategies of wood. PLOS Genetics 10(12): e1004759. doi:10.1371/journal.pgen.1004759 81. KERIÖ S, NIEMI S, HAAPANEN M, DANIEL G AND ASIEGBU FO (2015). Infection of Picea abies clones with a homokaryotic isolate of Heterobasidion parviporum under field conditions. Canadian Journal of Forest Research 45: 226–234, dx.doi.org/10.1139/cjfr-2014-0247 82. KOVALCHUK A, KERIÖ S, JABER E, RAFFAELLO T, LORENZ W, RAJENDRA GHIMIRE, DEAN R, HOLOPAINEN JK AND ASIEGBU FO (2014). Activation of defence pathways in Scots pine bark after feeding by Pine Weevil (Hylobius abietis). BMC Genomics (in press). 83. SILVA X, TERHONEN E, SUN H, KASANEN R, HELIÖVAARA K, JALKANEN R AND ASIEGBU FO (2014). Comparative analyses of fungal biota carried by the pine shoot beetle (Tomicus piniperda L.) in Northern and Southern Finland. Scandinavian Journal of Forest Research (in press). 01/04/2015